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Original Article
- Basic science and research
- Therapeutic hypothermia reduces inflammation and oxidative stress in the liver after asphyxial cardiac arrest in rats
- Yoonsoo Park, Ji Hyeon Ahn, Tae-Kyeong Lee, Bora Kim, Hyun-Jin Tae, Joon Ha Park, Myoung Cheol Shin, Jun Hwi Cho, Moo-Ho Won
- Acute Crit Care. 2020;35(4):286-295. Published online November 30, 2020
- DOI: https://doi.org/10.4266/acc.2020.00304
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- 3 Web of Science
- 5 Crossref
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Abstract
PDF - Background
Few studies have evaluated the effects of hypothermia on cardiac arrest (CA)-induced liver damage. This study aimed to investigate the effects of hypothermic therapy on the liver in a rat model of asphyxial cardiac arrest (ACA).
Methods
Rats were subjected to 5-minute ACA followed by return of spontaneous circulation (RoSC). Body temperature was controlled at 33°C±0.5°C or 37°C±0.5°C for 4 hours after RoSC in the hypothermia group and normothermia group, respectively. Liver tissues in each group were collected at 6 hours, 12 hours, 1 day, and 2 days after RoSC. To examine hepatic inflammation, mast cells were stained with toluidine blue. Superoxide anion radical production was evaluated using dihydroethidium fluorescence straining and expression of endogenous antioxidants (superoxide dismutase 1 [SOD1] and SOD2) was examined using immunohistochemistry.
Results
There were significantly more mast cells in the livers of the normothermia group with ACA than in the hypothermia group with ACA. Gradual increase in superoxide anion radical production was found with time in the normothermia group with ACA, but production was significantly suppressed in the hypothermia group with ACA relative to the normothermia group with ACA. SOD1 and SOD2 levels were higher in the hypothermia group with ACA than in the normothermia group with ACA.
Conclusions
Experimental hypothermic treatment after ACA significantly inhibited inflammation and superoxide anion radical production in the rat liver, indicating that this treatment enhanced or maintained expression of antioxidants. Our findings suggest that hypothermic therapy after CA can reduce mast cell-mediated inflammation through regulation of oxidative stress and the expression of antioxidants in the liver. -
Citations
Citations to this article as recorded by
- Mechanisms of low-temperature rehabilitation technologies. Natural and artificial hypothermia
Oleg A. Shevelev, Marina V. Petrova, Elias M. Mengistu, Vladislav A. Yakimenko, Darina N. Menzhurenkova, Irina N. Kolbaskina, Maria A. Zhdanova, Nadezhda A. Khodorovich, Ekaterina O. Sheveleva
Physical and rehabilitation medicine, medical rehabilitation.2023; 5(2): 141. CrossRef - Continuously increased generation of ROS in human plasma after cardiac arrest as determined by Amplex Red oxidation
Muhammad Shoaib, Nancy Kim, Rishabh C. Choudhary, Blanca Espin, Mitsuaki Nishikimi, Ann Iverson, Tsukasa Yagi, Seyedeh Shadafarin Marashi Shoshtari, Koichiro Shinozaki, Lance B. Becker, Junhwan Kim
Free Radical Research.2023; 57(5): 384. CrossRef - Prevention and correction of postdecompression liver dysfunction in obstructive jaundice in experimental animals
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Bulletin of the Medical Institute "REAVIZ" (REHABILITATION, DOCTOR AND HEALTH).2021; 11(4): 45. CrossRef - Hypothermic treatment reduces matrix metalloproteinase-9 expression and damage in the liver following asphyxial cardiac arrest in rats
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Laboratory Animal Research.2021;[Epub] CrossRef - High Oxygen Does Not Increase Reperfusion Injury Assessed with Lipid Peroxidation Biomarkers after Cardiac Arrest: A Post Hoc Analysis of the COMACARE Trial
Jaana Humaloja, Maximo Vento, Julia Kuligowski, Sture Andersson, José David Piñeiro-Ramos, Ángel Sánchez-Illana, Erik Litonius, Pekka Jakkula, Johanna Hästbacka, Stepani Bendel, Marjaana Tiainen, Matti Reinikainen, Markus B. Skrifvars
Journal of Clinical Medicine.2021; 10(18): 4226. CrossRef
- Mechanisms of low-temperature rehabilitation technologies. Natural and artificial hypothermia
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